Tatiana Zyri(Y)Anova as a highly motivated and capable stem cell researcher and passionate about finding therapeutic approaches to cure muscle and eye associated human diseases. Trained as both cell physiologist and molecular biologist.
Tatiana Zyri(Y)Anova, always aim to investigate the functional outcome of the problem applying cutting-age molecular biology tools. Particularly strong proficiency in ion channel biology and electrophysiology including “whole-cell” patch-clamp, primary cell isolation by FACS, molecular cloning using CRISPR/Cas9 mutagenesis, time-lapse confocal imaging (multiphoton, bioluminescence, Ca-). Excellent communication skills, leadership and teamwork experience from managing/training high school interns, undergraduate students, postdoctoral fellows and technical assistants, organizing lab events. Superior analytical and problem-solving, scientific writing skills.
- 2003–2007: Bachelor of Science (B.S.) degree St. Petersburg State University, St. Petersburg, Russia Advisor: Dr. Tatiana V. Matveeva.
- 2004–2007: Undergraduate Internship in Genetics/Biotechnology St. Petersburg State University, St. Petersburg, Russia Advisor: Dr. Tatiana V. Matveeva.
- 2007–2009: Master of Science (M.S.) degree St. Petersburg State University, St. Petersburg, Russia Advisors: Dr. Alexandr G. Markov, Dr. Natalia G. Pavlova.
- 2007: Undergraduate Internship in Physiology D. O. Ott Research Institute of Obstetrics and Gynecology St Petersburg, Russia Laboratory of Physiology and Pathophysiology of Fetus Advisor: Dr. Natalia G. Pavlova.
- 2008: Summer Internship in Ca2+–imaging technique Leipzig University, Germany Departments of Surgery/Traumatology and Urology Advisor: Dr. Jochen Neuhaus.
- 2011–2012: Predoctoral Fellowship Leipzig University, Germany. Departments of Surgery/Traumatology and Urology
- Advisors: Dr. Jochen Neuhaus, Dr. Gabriela Aust.
- 2013: Graduate Student Internship in Skeletal Muscle Ca2+–imaging Leipzig University, Germany Departments of Surgery/Traumatology and Urology Advisor: Dr. Jochen Neuhaus.
- 2009–2014: Doctor of Philosophy (Ph.D.) degree St. Petersburg State University, St. Petersburg, Russia Advisor: Dr. Alexandr G. Markov.
- 2014–2015: Postdoctoral Fellow in Electrophysiology Almazov Federal Heart, Blood and Endocrinology Center, St. Petersburg, Russia Advisor: Dr. Alexey V. Karpushev.
- 2015–2017: Postdoctoral Fellow in Molecular/Cell Biology Max Delbrueck Center for Molecular Medicine (MDC), Berlin, Germany Advisor: Dr. Carmen Birchmeier.
- 2015–2017: Postdoctoral Fellow in Molecular/Stem Cell Biology Max–Delbrueck Center for Molecular Medicine (MDC), Berlin, Germany
- Advisor: Dr. Carmen Birchmeier.
- 2017–2019: Postdoctoral Fellow in Molecular Medicine The Scripps Research Institute (Scripps Research), La Jolla, CA Advisor: Dr. Helen Makarenkova.
- 2019: Assistant Project Scientist in Pulmonary Physiology University of California Los Angeles (UCLA) Advisor: Dr. Andreas Schwingshackl.
- Zyrianova, T., Lopez, B., Liao, A., Gu, C., Schwingshackl, A. BK Channels Regulate TNFα-induced CCL-2 Release from Human Pulmonary Endothelial Cells. Am J Respir Cell Mol Biol.
- Pigichka, T. Y., Matveeva, T. V., Pavlova, O. I., and Lutova, L. A. (2006) Characterization of Nicotiana langsdorffii and Nicotiana rustica by the ability to agrobacterium wild type strain transformation. IV Russian Biotechnological Congress dedicated to U.A. Ovchinnikov. Pushchino, Russia (2006).
- Pigichka, T. Y., Pavlova, O. I., Matveeva, T. V., and Lutova, L. A. Characteristics of Nicotiana langsdorffii ability to regeneration and transformation in vitro. Biology is the 21st century science. 10th Pushchino school-conference for young scientists dedicated to the 50th anniversary of the Pushchino Scientific Center RA. Pushchino, Russia (2006).
- Pavlova, O. I., Pigichka, T. Y., Matveeva, T. V., and Lutova, L. A. Comparative characteristics of regenerative ability of Nicotiana rustica and Nicotiana langsdorffii, Genetics in Russia and globally. Moscow, Russia (2006).
- Markov A. G., Pigichka T. Y., Habulava N. V. Investigation of tight junction proteins in the syncytiotrophoblast of placenta. The functional mechanisms of visceral systems conference. St. Petersburg. Russia (2008).
- Pigichka, T. Y., Markov, A. G., and Pavlova N.G. Characteristics of hemohorial placenta. Mechanisms of visceral system functions. Russian Academy of Science dedicated to the 160th anniversary of the birthday of Pavlov I.P.). St. Petersburg, Russia (2009).
- Pigichka, T. Y. Immuno- and electrophysiological study of placental syncytiotrophoblast. Fundamental Science and Medicine. St. Petersburg, Russia (2009).
- Zyryanova, T.Y. CD97 is in fast- and slow-twitch fibers of human m. spinalis and m. vastus medialis. Human and Animal Physiology: From experiment to a clinical practice. XI Russian young scientist conference of Physiological Institute of Russian Academy of Science. Syktyvkar, Russia (2012).
- Zyryanova, T.Y. Intensity of CD97 expression in human skeletal muscle fibers. Biology is the 21st century science. 17th Pushchino school-conference of young scientists. Pushchino, Russia (2012).
- Zyryanova, T.Y. Investigation of CD97 influence on morphological and functional parameters and quantity of fast- and slow-twitch fibers in mouse m. soleus. Actual questions of medical science. Russian scientific and practically applied conference for students and young scientists, dedicated to the 70th anniversary of Prof. A.A. Chumakov (2012).
- Zyryanova, T.Y. Histological and biochemical phenotype parameters of skeletal muscles in wild type and CD97-knockout mice. Biology is the 21st century science. 18th Pushchino school-conference of young scientists. Pushchino, Russia (2013).
- Zyryanova, T. Y., Sittig, D., Schneider, R., Adams, V., Aust, G. CD97 in skeletal muscle. The 6th International Adhesion-GPCR Workshop. Wuerzburg, Germany (2014).
- Zyrianova, T., Bröhl, D., Lahmann, I., Ruffault, P.-L., Birchmeier, C. Oscillatory Activity of MyoD in Satellite Cells. ZING Conferences. Cell Fate Diversity in Aging. Dubrovnik, Croatia (2016).
- Zyrianova, T, Basova, L., Shah, M., Hamm-Alvarez, S., Makarenkova, H. Myoepithelial cell-driven acini contraction is impaired in mouse lacrimal glands during aging and inflammation. 2nd Annual Symposium. La Jolla Aging Meeting. Salk Institute, La Jolla, CA, (2018).
- Zyrianova, T., Basova, L., Chang, K., Umazume, T. and Makarenkova, H. The Role of Aging and Inflammation in Myoepithelial Cell and Lacrimal Gland Dysfunction. Molecular Medicine Department Retreat. The Scripps Research Institute. San Diego, CA (2018).
- Suarez-Berumen, K., Zyrianova, T., Collins-Hooper, H., Gromova, A., Meech, R., Sacco A., Dash, P.R., Mitchell, R., Shestopalov, V.I., Woolley, T.E., Patel, K., Makarenkova, H.P. Pannexin 1 regulates skeletal muscle regeneration by promoting bleb-based myoblast migration and fusion through a novel lipid based signaling mechanism. American Society of Cell Biology (ASCB)/European Molecular Biology Organization (EMBO) Annual Meeting, oral presentation at the minisymposium: Biology of Stem Cell, San Diego, CA (2018).
- Zyrianova, T., Lopez, B., Liao, A., Gu, C., Wong, L., Olcese, R., Ottolia, M., and Schwingshackl, A. Large conductance Ca2+-activated K+ channels regulate LPS-induced cytokine secretion from alveolar epithelial and endothelial cells. Biophysical Society 64th Annual Meeting, oral presentation at the platform: Membrane Receptors and Signal Transduction. San Diego, CA (2019).
- Liao A., Zyrianova, T., Gu, C., Lopez, B., Wong, L., Ottolia, M., Olcese, R., and Schwingshackl, A. Large conductance potassium channels are a new tool to regulate LPS-induced CCL-2 cytokine release from human pulmonary microvascular endothelial cells. UCLA Undergraduate Research Week, Los Angeles, CA (2020).
- Talapaneni, S., Wong, L., Gu, C., Zyrianova, T., Lopez, B., Olcese, R., Schwingshackl, A. Activation of TREK-1 potassium channels protects from hyperoxia-induced lung injury. UCLA Undergraduate Research Week, Los Angeles, CA (2020).
- Zyrianova, T., Lopez, B., Wong, L., Talapaneni, S., Gu, C., Olcese, R., Minor, D.L., Waters, C.M., and Schwingshackl, A. Activation of TREK-1 potassium channels protects from hyperoxia-induced lung injury. American Thoracic Society (ATS) 2020 International Conference. Philadelphia, (2020). Postponed due to COVID-20.
- Zyrianova, T., Lopez, B., Liao, A., Gu, C., Talapaneni, S., Schwingshackl, A. Activation of large conductance Ca2+-Activated K+ channels decreases TNF-α-induced CCL-2 secretion from human pulmonary microvascular endothelial cells. American Thoracic Society (ATS) 2021 International Conference. San Diego, CA (2021).
- Travel Award to attend the 6th Workshop on Adhesion-GPCRs in Wurzburg, Germany, Russian Foundation of Fundamental Researches, Moscow, Russia, (2012).
- Travel award to attend the 6th Workshop on Adhesion-GPCRs in Wurzburg, Germany St. Petersburg State University. St. Petersburg, Russia, (2012).
- Postdoctoral Mobility Grant to attend Research Opportunities Week at Technical University of Munich (TUM), Talent Factory of Technical University of Munich (TUM), Munich, Germany, (2014).
- Best Poster Award, the 2nd place, 2nd Annual Symposium “La Jolla Aging Meeting”. Salk Institute, La Jolla, CA, (2018).
- Best talk, the 2nd place, Postdoc Lightning Talks, the Scripps Research Institute, La Jolla, CA, (2018).
- Leica Special Project Award to perform ex vivo dual multiphoton imaging at La Jolla Institute for Immunology (LJI), La Jolla, CA, (2018).
- Ca-signaling in Skeletal Muscle. Summer seminars. Leipzig University, Leipzig, Germany, (2009).
- The role of CD97 in Skeletal Muscle. Institute for Diabetes and Obesity, German Research Center for the Environmental health and Technical University of Munich, Munich, Germany, (2014).
- The role of CD97 in Skeletal Muscle. Department of Neuroscience. Max-Delbrueck Center for Molecular Medicine (MDC), Berlin, Germany, (2015).
- Oscillatory Activity of MyoD in Satellite Cells. Muscle Seminars. San Diego Muscle Research Center (SDMRC). La Jolla, CA, (2017).
- Lacrimal Gland Function during Aging and Disease. Postdoc Open Mic Night. La Jolla, CA (2018).
- Dry Eye Disease: the Effect of Aging in Myoepithelial Cell and Lacrimal Gland Dysfunction. Postdoc Lightning Talks, the Scripps Research Institute, La Jolla, CA, (2018).
- From Ion Channels to Stem Cells. Department of Pediatrics. UCLA. Los Angeles, CA, (2018).
- From Ion Channels to Stem Cells. Gillespie Neuroscience Research Facility. University of California Irvine, Irvine, CA, (2019).
- From Ion Channels to Stem Cells. Department of Physiology & Biomedical Engineering. Mayo Clinic, Rochester, MN, (2019).
